CUFFCOMPARE(1)

NAME
cuffcompare - helps analyze the transfrags

SYNOPSIS
cuffcompare [-r <reference_mrna.gtf>] [-R] [-T] [-V] [-s <seq_path>]

    [-o <outprefix>] [-p <cprefix>] {-i <input_gtf_list> | <input1.gtf> [<input2.gtf> .. <inputN.gtf>]}

DESCRIPTION
Cuffcompare provides classification, reference annotation mapping and various statistics for Cufflinks transfrags. Cuffcompare clusters and tracks transfrags across multiple samples, writing matching transcripts (intron chains) into <outprefix>.tracking, and a GTF file <outprefix>.combined.gtf containing a nonredundant set of transcripts across all input files (with a single representative transfrag chosen for each clique of matching transfrags across samples).

OPTIONS
-i provide a text file with a list of Cufflinks GTF files to process instead

    of expecting them as command line arguments (useful when a large number of GTF files should be processed)

-r a set of known mRNAs to use as a reference for assessing

    the accuracy of mRNAs or gene models given in <input.gtf>

-R for -r option, reduce the set of reference transcripts to

    only those found to overlap any of the input loci

-M discard (ignore) single-exon transfrags and reference transcripts -N discard (ignore) single-exon reference transcripts

-s <seq_path> can be a multi-fasta file with all the genomic sequences or

    a directory containing multiple single-fasta files (one file per contig); lower case bases will be used to classify input transcripts as repeats

-d max distance (range) for grouping transcript start sites (100) -p the name prefix to use for consensus transcripts in the

    <outprefix>.combined.gtf file (default: 'TCONS')

-C include the "contained" transcripts in the .combined.gtf file -G generic GFF input file(s) (do not assume Cufflinks GTF) -T do not generate .tmap and .refmap files for each input file -V verbose processing mode (showing all GFF parsing warnings)

SEE ALSO
http://cufflinks.cbcb.umd.edu/manual.html#cuffcompare