CUFFLINKS:(1)

NAME
cufflinks - Transcript assembly, differential expression, and differential regulation for RNA-Seq

DESCRIPTION
see online page http://cufflinks.cbcb.umd.edu/manual.html

SYNOPSIS
cufflinks [options] <hits.sam>

OPTIONS

-o/--output-dir
    write all output files to this directory [ default: ./ ]

-p/--num-threads
    number of threads used during analysis [ default: 1 ]

-G/--GTF
    quantitate against reference transcript annotations

-g/--GTF-guide
    use reference transcript annotation to guide assembly

-M/--mask-file
    ignore all alignment within transcripts in this file

-b/--frag-bias-correct
    use bias correction - reference fasta required [ default: NULL ]

-u/--multi-read-correct
    use 'rescue method' for multi-reads (more accurate) [ default: FALSE ]

--library-type
    library prep used for input reads [ default: below ]

  Advanced Abundance Estimation Options:

-m/--frag-len-mean
    average fragment length (unpaired reads only) [ default: 200 ]

-s/--frag-len-std-dev
    fragment length std deviation (unpaired reads only) [ default: 80 ]

--upper-quartile-norm
    use upper-quartile normalization [ default: FALSE ]

--max-mle-iterations
    maximum iterations allowed for MLE calculation [ default: 5000 ]

--num-importance-samples
    number of importance samples for MAP restimation [ default: 1000 ]

--compatible-hits-norm
    count hits compatible with reference RNAs only [ default: FALSE ]

--total-hits-norm
    count all hits for normalization [ default: TRUE ]

  Advanced Assembly Options:

-L/--label
    assembled transcripts have this ID prefix [ default: CUFF ]

-F/--min-isoform-fraction
    suppress transcripts below this abundance level [ default: 0.10 ]

-j/--pre-mrna-fraction
    suppress intra-intronic transcripts below this level [ default: 0.15 ]

-I/--max-intron-length
    ignore alignments with gaps longer than this [ default: 300000 ]

-a/--junc-alpha
    alpha for junction binomial test filter [ default: 0.001 ]

-A/--small-anchor-fraction
    percent read overhang taken as 'suspiciously small' [ default: 0.09 ]

--min-frags-per-transfrag
    minimum number of fragments needed for new transfrags [ default: 10 ]

--overhang-tolerance
    number of terminal exon bp to tolerate in introns [ default: 8 ]

--max-bundle-length
    maximum genomic length allowed for a given bundle [ default:3500000 ]

--min-intron-length
    minimum intron size allowed in genome [ default: 50 ]

--trim-3-avgcov-thresh
    minimum avg coverage required to attempt 3' trimming [ default: 10 ]

--trim-3-dropoff-frac
    fraction of avg coverage below which to trim 3' end [ default: 0.1 ]

  Advanced Reference Annotation Guided Assembly Options:

--no-faux-reads
    disable tiling by faux reads [ default: FALSE ]

--3-overhang-tolerance
    overhang allowed on 3' end when merging with reference[ default: 600 ]

--intron-overhang-tolerance
    overhang allowed inside reference intron when merging [ default: 30 ]

  Advanced Program Behavior Options:

-v/--verbose
    log-friendly verbose processing (no progress bar) [ default: FALSE ]

-q/--quiet
    log-friendly quiet processing (no progress bar) [ default: FALSE ]

--no-update-check
    do not contact server to check for update availability[ default: FALSE ]

  Supported library types:

    ff-firststrand ff-secondstrand ff-unstranded fr-firststrand fr-secondstrand fr-unstranded (default) transfrags

cufflinks v1.0.2 linked against Boost version 104601 ----------------------------- Usage: cufflinks [options] <hits.sam> General Options:

-o/--output-dir
    write all output files to this directory [ default: ./ ]

-p/--num-threads
    number of threads used during analysis [ default: 1 ]

-G/--GTF
    quantitate against reference transcript annotations

-g/--GTF-guide
    use reference transcript annotation to guide assembly

-M/--mask-file
    ignore all alignment within transcripts in this file

-b/--frag-bias-correct
    use bias correction - reference fasta required [ default: NULL ]

-u/--multi-read-correct
    use 'rescue method' for multi-reads (more accurate) [ default: FALSE ]

--library-type
    library prep used for input reads [ default: below ]

  Advanced Abundance Estimation Options:

-m/--frag-len-mean
    average fragment length (unpaired reads only) [ default: 200 ]

-s/--frag-len-std-dev
    fragment length std deviation (unpaired reads only) [ default: 80 ]

--upper-quartile-norm
    use upper-quartile normalization [ default: FALSE ]

--max-mle-iterations
    maximum iterations allowed for MLE calculation [ default: 5000 ]

--num-importance-samples
    number of importance samples for MAP restimation [ default: 1000 ]

--compatible-hits-norm
    count hits compatible with reference RNAs only [ default: FALSE ]

--total-hits-norm
    count all hits for normalization [ default: TRUE ]

  Advanced Assembly Options:

-L/--label
    assembled transcripts have this ID prefix [ default: CUFF ]

-F/--min-isoform-fraction
    suppress transcripts below this abundance level [ default: 0.10 ]

-j/--pre-mrna-fraction
    suppress intra-intronic transcripts below this level [ default: 0.15 ]

-I/--max-intron-length
    ignore alignments with gaps longer than this [ default: 300000 ]

-a/--junc-alpha
    alpha for junction binomial test filter [ default: 0.001 ]

-A/--small-anchor-fraction
    percent read overhang taken as 'suspiciously small' [ default: 0.09 ]

--min-frags-per-transfrag
    minimum number of fragments needed for new transfrags [ default: 10 ]

--overhang-tolerance
    number of terminal exon bp to tolerate in introns [ default: 8 ]

--max-bundle-length
    maximum genomic length allowed for a given bundle [ default:3500000 ]

--min-intron-length
    minimum intron size allowed in genome [ default: 50 ]

--trim-3-avgcov-thresh
    minimum avg coverage required to attempt 3' trimming [ default: 10 ]

--trim-3-dropoff-frac
    fraction of avg coverage below which to trim 3' end [ default: 0.1 ]

  Advanced Reference Annotation Guided Assembly Options:

--no-faux-reads
    disable tiling by faux reads [ default: FALSE ]

--3-overhang-tolerance
    overhang allowed on 3' end when merging with reference[ default: 600 ]

--intron-overhang-tolerance
    overhang allowed inside reference intron when merging [ default: 30 ]

  Advanced Program Behavior Options:

-v/--verbose
    log-friendly verbose processing (no progress bar) [ default: FALSE ]

-q/--quiet
    log-friendly quiet processing (no progress bar) [ default: FALSE ]

--no-update-check
    do not contact server to check for update availability[ default: FALSE ]

  Supported library types:

    ff-firststrand ff-secondstrand ff-unstranded fr-firststrand fr-secondstrand fr-unstranded (default) transfrags