ID   CASB_HUMAN              Reviewed;         226 AA.
AC   P05814; Q4VAZ9; Q9UCM5;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 4.
DT   03-MAR-2009, entry version 86.
DE   RecName: Full=Beta-casein;
DE   Flags: Precursor;
GN   Name=CSN2; Synonyms=CASB;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=90353560; PubMed=2387396; DOI=10.1016/0014-5793(90)81142-B;
RA   Loennerdal B., Bergstroem S., Andersson Y., Hjalmarsson K.,
RA   Sundqvist A.K., Hernell O.;
RT   "Cloning and sequencing of a cDNA encoding human milk beta-casein.";
RL   FEBS Lett. 269:153-156(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Placenta;
RX   MEDLINE=94156198; PubMed=8112603; DOI=10.1016/0378-1119(94)90754-4;
RA   Hansson L., Edlund A., Johansson T., Hernell O., Stroemqvist M.,
RA   Lindquist S., Loennerdal B., Bergstroem S.;
RT   "Structure of the human beta-casein encoding gene.";
RL   Gene 139:193-199(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Mammary gland;
RA   Menon R.S.;
RL   Submitted (OCT-1989) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Placenta;
RA   Kwiatkowski D.J.;
RT   "A high resolution linkage map of human 9q34.1.";
RL   Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   PROTEIN SEQUENCE OF 16-226, AND PHOSPHORYLATION AT THR-18; SER-21;
RP   SER-23; SER-24 AND SER-25.
RX   MEDLINE=84185624; PubMed=6715339;
RA   Greenberg R., Groves M.L., Dower H.J.;
RT   "Human beta-casein. Amino acid sequence and identification of
RT   phosphorylation sites.";
RL   J. Biol. Chem. 259:5132-5138(1984).
RN   [7]
RP   PROTEIN SEQUENCE OF 18-33, PHOSPHORYLATION AT THR-18; SER-21; SER-23;
RP   SER-24 AND SER-25, AND MASS SPECTROMETRY.
RX   PubMed=18847231; DOI=10.1021/pr800387s;
RA   Poth A.G., Deeth H.C., Alewood P.F., Holland J.W.;
RT   "Analysis of the human casein phosphoproteome by 2-D electrophoresis
RT   and MALDI-TOF/TOF MS reveals new phosphoforms.";
RL   J. Proteome Res. 7:5017-5027(2008).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 161-226.
RC   TISSUE=Mammary gland;
RX   MEDLINE=89240053; PubMed=2717418; DOI=10.1093/nar/17.7.2869;
RA   Menon R.S., Ham R.G.;
RT   "Human beta-casein: partial cDNA sequence and apparent polymorphism.";
RL   Nucleic Acids Res. 17:2869-2869(1989).
RN   [9]
RP   PROTEIN SEQUENCE OF 176-215.
RX   PubMed=1369431;
RA   Azuma N., Yamauchi K.;
RT   "Plasmin cleavage of human beta-casein.";
RL   Biosci. Biotechnol. Biochem. 56:1140-1141(1992).
CC   -!- FUNCTION: Important role in determination of the surface
CC       properties of the casein micelles.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
CC   -!- PTM: Form 1-P is phosphorylated once; half of the molecules are
CC       phosphorylated on Ser-24, half on Ser-25.
CC   -!- SIMILARITY: Belongs to the beta-casein family.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Of buttons, digestion
CC       and glue - Issue 16 of November 2001;
CC       URL="http://www.expasy.org/spotlight/back_issues/sptlt016.shtml";
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DR   EMBL; X55739; CAA39270.1; -; mRNA.
DR   EMBL; AF027807; AAC82978.1; -; Genomic_DNA.
DR   EMBL; X17070; CAA34916.1; -; mRNA.
DR   EMBL; BC069554; AAH69554.1; -; mRNA.
DR   EMBL; BC096194; AAH96194.1; -; mRNA.
DR   EMBL; BC096195; AAH96195.1; -; mRNA.
DR   EMBL; BC096196; AAH96196.1; -; mRNA.
DR   EMBL; BC096197; AAH96197.1; -; mRNA.
DR   EMBL; X13766; CAA32017.1; -; mRNA.
DR   IPI; IPI00010849; -.
DR   PIR; I53730; KBHU.
DR   RefSeq; NP_001882.1; -.
DR   UniGene; Hs.2242; -.
DR   DisProt; DP00199; -.
DR   DIP; DIP:528N; -.
DR   PhosphoSite; P05814; -.
DR   PRIDE; P05814; -.
DR   Ensembl; ENSG00000135222; Homo sapiens.
DR   GeneID; 1447; -.
DR   KEGG; hsa:1447; -.
DR   GeneCards; GC04M070869; -.
DR   H-InvDB; HIX0031475; -.
DR   HGNC; HGNC:2447; CSN2.
DR   MIM; 115460; gene.
DR   PharmGKB; PA26950; -.
DR   HOGENOM; P05814; -.
DR   HOVERGEN; P05814; -.
DR   Pathway_Interaction_DB; ptp1bpathway; Signaling events mediated by PTP1B.
DR   NextBio; 5947; -.
DR   ArrayExpress; P05814; -.
DR   Bgee; P05814; -.
DR   CleanEx; HS_CSN2; -.
DR   GermOnline; ENSG00000135222; Homo sapiens.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0005509; F:calcium ion binding; TAS:ProtInc.
DR   GO; GO:0004857; F:enzyme inhibitor activity; TAS:ProtInc.
DR   GO; GO:0005215; F:transporter activity; IEA:InterPro.
DR   GO; GO:0006816; P:calcium ion transport; TAS:ProtInc.
DR   InterPro; IPR001588; Casein.
DR   InterPro; IPR016345; Casein_beta.
DR   Pfam; PF00363; Casein; 1.
DR   PIRSF; PIRSF002372; Beta-casein; 1.
DR   PROSITE; PS00306; CASEIN_ALPHA_BETA; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Milk protein; Phosphoprotein; Secreted;
KW   Signal.
FT   SIGNAL        1     15
FT   CHAIN        16    226       Beta-casein.
FT                                /FTId=PRO_0000004475.
FT   MOD_RES      18     18       Phosphothreonine; in form 5-P.
FT   MOD_RES      21     21       Phosphoserine; in form 4-P and form 5-P.
FT   MOD_RES      23     23       Phosphoserine; in form 3-P, form 4-P and
FT                                form 5-P.
FT   MOD_RES      24     24       Phosphoserine; in form 1-P, form 2-P,
FT                                form 3-P, form 4-P and form 5-P.
FT   MOD_RES      25     25       Phosphoserine; in form 1-P, form 2-P,
FT                                form 3-P, form 4-P and form 5-P.
FT   CONFLICT     30     30       T -> P (in Ref. 6; AA sequence).
FT   CONFLICT     34     34       Missing (in Ref. 1; CAA39270).
FT   CONFLICT     48     50       EDE -> TDQ (in Ref. 6; AA sequence).
FT   CONFLICT    120    120       S -> Q (in Ref. 6; AA sequence).
FT   CONFLICT    133    133       L -> V (in Ref. 3; CAA34916).
FT   CONFLICT    140    140       H -> Q (in Ref. 3; CAA34916).
FT   CONFLICT    149    149       L -> S (in Ref. 6; AA sequence).
FT   CONFLICT    173    173       Q -> E (in Ref. 6; AA sequence).
FT   CONFLICT    182    184       QVV -> EVL (in Ref. 6; AA sequence).
FT   CONFLICT    188    188       Q -> V (in Ref. 6; AA sequence).
FT   CONFLICT    207    207       T -> P (in Ref. 6; AA sequence).
FT   CONFLICT    214    222       TQPLAPVHN -> PEPSTTZABH (in Ref. 6; AA
FT                                sequence).
SQ   SEQUENCE   226 AA;  25382 MW;  2619C524EA1358E8 CRC64;
     MKVLILACLV ALALARETIE SLSSSEESIT EYKQKVEKVK HEDQQQGEDE HQDKIYPSFQ
     PQPLIYPFVE PIPYGFLPQN ILPLAQPAVV LPVPQPEIME VPKAKDTVYT KGRVMPVLKS
     PTIPFFDPQI PKLTDLENLH LPLPLLQPLM QQVPQPIPQT LALPPQPLWS VPQPKVLPIP
     QQVVPYPQRA VPVQALLLNQ ELLLNPTHQI YPVTQPLAPV HNPISV
//
