>CCGCCCTTGCCCTTCCTCCCTTCCCTTTCGGAGTCCTGGCCCCACCCTGT	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	0 0  perfect match
 CCGCCCTTGCCCTTCCTCCCTTCCCTTTCGGAGTCCTGGCCCCACCCTGT	1..50	+synth1:31..80	start:0..end:0,matches:50,sub:0	segs:1,align_score:0,mapq:26

>ACAGGGTGGGGCCAGGACTCCGAAAGGGAAGGGAGGAAGGGCAAGGGCGG	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	1 1  perfect match on reverse strand
 ACAGGGTGGGGCCAGGACTCCGAAAGGGAAGGGAGGAAGGGCAAGGGCGG	1..50	-synth1:80..31	start:0..end:0,matches:50,sub:0	segs:1,align_score:0,mapq:26

>CCGCCCTTGCCCTTCCTCCCTTCCCTCGTGGAGTCCTGGCCCCACCCTGT	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	2 2  mutation
 CCGCCCTTGCCCTTCCTCCCTTCCCTttcGGAGTCCTGGCCCCACCCTGT	1..50	+synth1:31..80	start:0..end:0,matches:47,sub:3	segs:1,align_score:3,mapq:26

>ACAGGGTGGGGCCAGGACTCCGAAAGTAGAGGGAGGAAGGGCAAGGGCGG	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	3 3  mutation on reverse strand
 ACAGGGTGGGGCCAGGACTCCGAAAGggaAGGGAGGAAGGGCAAGGGCGG	1..50	-synth1:80..31	start:0..end:0,matches:47,sub:3	segs:1,align_score:3,mapq:26

>CCGCCCTTGCCCTTCCTCCCTTCCCTATCTTCGGAGTCCTGGCCCCACCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	4 4  insertion
 CCGCCCTTGCCCTTCCTCCCTTCCCT------------------------	1..26	+synth1:31..56	start:0..ins:3,matches:26,sub:0	segs:2,align_score:2,mapq:26
,-----------------------------TTCGGAGTCCTGGCCCCACCC	30..50	+synth1:57..77	ins:3..end:0,matches:21,sub:0

>ACAGGGTGGGGCCAGGACTCCGAAAGATCGGAAGGGAGGAAGGGCAAGGG	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	5 5  insertion on reverse strand
 ACAGGGTGGGGCCAGGACTCCGAAAG------------------------	1..26	-synth1:80..55	start:0..ins:3,matches:26,sub:0	segs:2,align_score:2,mapq:26
,-----------------------------GGAAGGGAGGAAGGGCAAGGG	30..50	-synth1:54..34	ins:3..end:0,matches:21,sub:0

>CCGCCCTTGCCCTTCCTCCCTTCCCTGGAGTCCTGGCCCCACCCTGT	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456	6 6  deletion
 CCGCCCTTGCCCTTCCTCCCTTCCCTttc---------------------	1..26	+synth1:31..56	start:0..del:3,matches:26,sub:0	segs:2,align_score:2,mapq:26
,--------------------------GGAGTCCTGGCCCCACCCTGT	27..47	+synth1:60..80	del:3..end:0,matches:21,sub:0

>ACAGGGTGGGGCCAGGACTCCGAAAGAGGGAGGAAGGGCAAGGGCGG	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456	7 7  deletion on reverse strand
 ACAGGGTGGGGCCAGGACTCCGAAAGgga---------------------	1..26	-synth1:80..55	start:0..del:3,matches:26,sub:0	segs:2,align_score:2,mapq:26
,--------------------------AGGGAGGAAGGGCAAGGGCGG	27..47	-synth1:51..31	del:3..end:0,matches:21,sub:0

>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	8 8 perfect start of ref
 CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1..50	+synth1:1..50	start:0..end:0,matches:50,sub:0	segs:1,align_score:0,mapq:26

>GGGAGGAAGGGCAAGGGCGGCCGCGGGCACTGGGTCCAGCCCTGCCTCAG	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	9 9 perfect start of ref reverse strand
 GGGAGGAAGGGCAAGGGCGGCCGCGGGCACTGGGTCCAGCCCTGCCTCAG	1..50	-synth1:50..1	start:0..end:0,matches:50,sub:0	segs:1,align_score:0,mapq:26

>GAGTCCTGGCCCCACCCTGTGCTTCCCCTCCGCCTGCTGCACAGGCGCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	10 10 perfect end of ref
 GAGTCCTGGCCCCACCCTGTGCTTCCCCTCCGCCTGCTGCACAGGCGCCC	1..50	+synth1:61..110	start:0..end:0,matches:50,sub:0	segs:1,align_score:0,mapq:26

>GGGCGCCTGTGCAGCAGGCGGAGGGGAAGCACAGGGTGGGGCCAGGACTC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	11 11 perfect end of ref reverse strand
 GGGCGCCTGTGCAGCAGGCGGAGGGGAAGCACAGGGTGGGGCCAGGACTC	1..50	-synth1:110..61	start:0..end:0,matches:50,sub:0	segs:1,align_score:0,mapq:26

>ATGAGGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	12 12 mismatch at beginning x1, starting at 1, with mutation at 20
 cTGAGGCAGGGCTGGACCCaGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	2..50	+synth1:2..50	start:1..end:0,matches:48,sub:1	segs:1,align_score:2,mapq:26

>AAGAGGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	13 13 mismatch at beginning x2, starting at 1, with mutation at 20
 ctGAGGCAGGGCTGGACCCaGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	3..50	+synth1:3..50	start:2..end:0,matches:47,sub:1	segs:1,align_score:3,mapq:26

>AAAAAGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	14 14 mismatch at beginning x5, starting at 1 (pos 4 actually does match), with mutation at 20
 ctgAgGCAGGGCTGGACCCaGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	6..50	+synth1:6..50	start:5..end:0,matches:45,sub:1	segs:1,align_score:5,mapq:26

>CAGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	15 15 mismatch at beginning x1, starting at 2
 CtGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	3..50	+synth1:3..50	start:2..end:0,matches:49,sub:0	segs:1,align_score:1,mapq:26

>CAAAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	16 16 mismatch at beginning x2, starting at 2
 CtgAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	4..50	+synth1:4..50	start:3..end:0,matches:48,sub:0	segs:1,align_score:2,mapq:26

>CAAAAACAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	17 17 mismatch at beginning x5, starting at 2 (pos 4 actually does match)
 CtgAggCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC	7..50	+synth1:7..50	start:6..end:0,matches:46,sub:0	segs:1,align_score:4,mapq:26

>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCA	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	18 18 mismatch at end x1, starting at -1
 CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCc	1..49	+synth1:1..49	start:0..end:1,matches:49,sub:0	segs:1,align_score:1,mapq:26

>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCAA	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	19 19 mismatch at end x2, starting at -1
 CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCcc	1..48	+synth1:1..48	start:0..end:2,matches:48,sub:0	segs:1,align_score:2,mapq:26

>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCAAAAA	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	20 20 mismatch at end x5, starting at -1
 CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCctccc	1..45	+synth1:1..45	start:0..end:5,matches:45,sub:0	segs:1,align_score:5,mapq:26

>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCAC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	21 21 mismatch at end x1, starting at -2
 CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCcC	1..48	+synth1:1..48	start:0..end:2,matches:49,sub:0	segs:1,align_score:1,mapq:26

>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTAAC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	22 22 mismatch at end x2, starting at -2
 CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTccC	1..47	+synth1:1..47	start:0..end:3,matches:48,sub:0	segs:1,align_score:2,mapq:26

>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTAAAAAC	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	23 23 mismatch at end x5, starting at -2
 CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTcctccC	1..44	+synth1:1..44	start:0..end:6,matches:45,sub:0	segs:1,align_score:5,mapq:26

>ACGCCCTTGCAATTCCTCCCTTCCCTTTCGGCCTGGCCCCACCCTGA	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456	24 24 padding left & right, mutation, deletion
 cCGCCCTTGCccTTCCTCCCTTCCCTTTCGGagt----------------	2..31	+synth1:32..61	start:1..del:3,matches:28,sub:2	segs:2,align_score:6,mapq:26
,-------------------------------CCTGGCCCCACCCTGt	32..46	+synth1:65..79	del:3..end:1,matches:15,sub:0

>ACGCCCTTGCAATTCCTCCCTTCCCTATCTTCGGAGTCCTGGCCCCACCA	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	25 25 padding left & right, mutation, insertion
 cCGCCCTTGCccTTCCTCCCTTCCCT------------------------	2..26	+synth1:32..56	start:1..ins:3,matches:23,sub:2	segs:2,align_score:6,mapq:26
,-----------------------------TTCGGAGTCCTGGCCCCACCc	30..49	+synth1:57..76	ins:3..end:1,matches:20,sub:0

>TGGTGGGGCCAGGACTCCGAAGATAGGGAAGGGAGGAATTGCAAGGGCGT	1	"#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789	26 26 padding left & right, mutation, insertion, reverse complemented
 gGGTGGGGCCAGGACTCCGAA-----------------------------	2..21	-synth1:76..57	start:1..ins:3,matches:20,sub:0	segs:2,align_score:6,mapq:26
,------------------------AGGGAAGGGAGGAAggGCAAGGGCGg	25..49	-synth1:56..32	ins:3..end:1,matches:23,sub:2

