@0  perfect match
CCGCCCTTGCCCTTCCTCCCTTCCCTTTCGGAGTCCTGGCCCCACCCTGT
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@1  perfect match on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAGGGAAGGGAGGAAGGGCAAGGGCGG
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@2  mutation
CCGCCCTTGCCCTTCCTCCCTTCCCTCGTGGAGTCCTGGCCCCACCCTGT
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@3  mutation on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAGTAGAGGGAGGAAGGGCAAGGGCGG
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@4  insertion
CCGCCCTTGCCCTTCCTCCCTTCCCTATCTTCGGAGTCCTGGCCCCACCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@5  insertion on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAGATCGGAAGGGAGGAAGGGCAAGGG
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@6  deletion
CCGCCCTTGCCCTTCCTCCCTTCCCTGGAGTCCTGGCCCCACCCTGT
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTU
@7  deletion on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAGAGGGAGGAAGGGCAAGGGCGG
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTU
@8 perfect start of ref
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@9 perfect start of ref reverse strand
GGGAGGAAGGGCAAGGGCGGCCGCGGGCACTGGGTCCAGCCCTGCCTCAG
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@10 perfect end of ref
GAGTCCTGGCCCCACCCTGTGCTTCCCCTCCGCCTGCTGCACAGGCGCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@11 perfect end of ref reverse strand
GGGCGCCTGTGCAGCAGGCGGAGGGGAAGCACAGGGTGGGGCCAGGACTC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@12 mismatch at beginning x1, starting at 1, with mutation at 20
ATGAGGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@13 mismatch at beginning x2, starting at 1, with mutation at 20
AAGAGGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@14 mismatch at beginning x5, starting at 1 (pos 4 actually does match), with mutation at 20
AAAAAGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@15 mismatch at beginning x1, starting at 2
CAGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@16 mismatch at beginning x2, starting at 2
CAAAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@17 mismatch at beginning x5, starting at 2 (pos 4 actually does match)
CAAAAACAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@18 mismatch at end x1, starting at -1
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCA
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@19 mismatch at end x2, starting at -1
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCAA
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@20 mismatch at end x5, starting at -1
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCAAAAA
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@21 mismatch at end x1, starting at -2
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCAC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@22 mismatch at end x2, starting at -2
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTAAC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@23 mismatch at end x5, starting at -2
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTAAAAAC
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@24 padding left & right, mutation, deletion
ACGCCCTTGCAATTCCTCCCTTCCCTTTCGGCCTGGCCCCACCCTGA
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTU
@25 padding left & right, mutation, insertion
ACGCCCTTGCAATTCCTCCCTTCCCTATCTTCGGAGTCCTGGCCCCACCA
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@26 padding left & right, mutation, insertion, reverse complemented
TGGTGGGGCCAGGACTCCGAAGATAGGGAAGGGAGGAATTGCAAGGGCGT
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTUVWX
@27 padding left & right, deletion then mutation
CCGCCCTTGCCCCTCCCTTCCCTTTCGGAGTCAAGGCCCCACCCTGT
+
ABCDEFGHIJKLMNOPQRSTUVWXYZABCDEFGHIJKLMNOPQRSTU

